Response of Functional Neutrophils after Exposure to a Novel ImmuneChip (Energy Cell)

Neutrophils are the most abundant type of granulocytes in most mammals. They possess a dual role as phagocytes and as proinflammatory cells [1]. By being present in the bloodstream, they form the first line of cellular defense against invading microbial pathogens as an essential part of the innate immune system. Moreover, neutrophils are the first responders of inflammatory cells which migrate towards the site of inflammation [2-6]. Similar to macrophages, neutrophils attack microbial pathogens by activating a so-called respiratory or oxidative burst which is independent from energy-producing metabolic processes and involves the catalytic conversion of dimolecular oxygen into superoxide anion radicals by the NADPH oxidase complex [7]. Secondarily, also other strong oxidizing agents with potent antimicrobial properties are generated. However, reactive oxygen species have been increasingly recognized as important components of intracellular signaling [8]. Prompted by this background we investigated the effect of a novel immune chip (= Energy Cell) on the growth characteristics and the oxidative burst of cultivated human promyelocytes which have been differentiated to functional neutrophils in corresponding cultures without and with exposure to the Energy Cell. Materials and Methods Description of the Energy Cell Basically, the Energy Cell is influenced by frequency spectra that are generated by special frequency generators. With the so-called vital field technology, electromagnetic fields (up to 120 GHz), microcurrent frequencies (up to 1 GHz) and different magnetic fields are generated via special antennas. The Energy Cell is placed on these antennas and exposed to the specific frequencies for a definite time period. Due to its storage properties, the chip is able to record this information and release it over several months. In the case of the Energy Cell, the stored frequencies of specific immune cells were used and the chip was exposed to this pattern in order to achieve the desired result. This patented information store is manufactured using a special process after years of development work. A precisely defined proportion of high-quality storage media is poured into a particularly skin-friendly silicone base. This makes it possible to conserve the effect of these frequency spectra as long as possible. Experience to actual data show that the chip remains fully effective for at least 6 months and should be replaced afterwards. Neutrophils are the most abundant type of granulocytes in most mammals. They possess a dual role as phagocytes and as proinflammatory cells. By being present in the bloodstream, they form the first line of cellular defense against invading microbial pathogens as an essential part of the innate immune system. The experiments were conducted with cultivated human promyelocytes which were directly exposed to a novel immune chip during their 6-day differentiation period to functional neutrophils. The same cells, but without the immune chip (= Energy Cell), served as corresponding controls. The samples were cultivated in the same incubator, but separated from each other by several layers of aluminum foil. After differentiation, the growth characteristics, and the oxidative burst of the functional neutrophils without and with exposure to the Energy Cell were examined. Altogether, 5 independent experiments on different days were conducted. In comparison to the untreated controls, the Energy Cell caused an increased cell number by 17.2 ± 3.8% and an increased cell diameter by 9.5 ± 0.2% (mean values ± standard deviations; p ≤ 0.05). Moreover, the basal cell metabolism of the functional neutrophils as well as the generation of superoxide anion radicals was also increased by 23.0 ± 5.5% and 21.3 ± 6.2%, respectively (mean values ± standard deviations; p ≤ 0.05). The results suggest that use of the Energy Cell might be able to cause an improved defense against microbial pathogens.