Key result
Optoporation applied to microelectrode arrays enables measuring action potentials from human-derived cardiac syncytia for more than 1 continuous month to detect chronic cardiotoxicity.
Why the study?
Existing in vitro screening techniques do not offer both high sensitivity for transmembrane ion currents and low invasiveness for long-term cell monitoring to identify chronic cardiotoxicity.
Laser optoporation on microelectrode arrays allows for non-invasive, long-term monitoring of cardiomyocyte action potentials, enabling the detection of chronic drug-induced cardiotoxicity in vitro.
Supports in vitro chronic cardiotoxicity screening; leaves open translation to clinical monitoring and human relevance.
The reliable identification of chronic cardiotoxic effects in in vitro screenings is fundamental for filtering out toxic molecular entities before in vivo animal experimentation and clinical trials. Present techniques such as patch-clamp, voltage indicators, and standard microelectrode arrays do not offer at the same time high sensitivity for measuring transmembrane ion currents and low-invasiveness for monitoring cells over long time. Here, we show that optoporation applied to microelectrode arrays enables measuring action potentials from human-derived cardiac syncytia for more than 1 continuous month and provides reliable data on chronic cardiotoxic effects caused by known compounds such as pentamidine. The technique has high potential for detecting chronic cardiotoxicity in the early phases of drug development.
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Iachetta et al. (2022) studied Cardiotoxicity. Cell optoporation on microelectrode arrays was evaluated on Action potential measurement duration and reliability. Optoporation applied to microelectrode arrays enables measuring action potentials from human-derived cardiac syncytia for more than 1 continuous month to detect chronic cardiotoxicity.
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