Key result
Recombinant APV/C fusion protein was recognized by both APV/C-specific and hMPV-specific antibodies, demonstrating significant antigenic cross-reactivity between the two viruses.
Demonstrates significant antigenic cross-reactivity between APV/C and hMPV fusion proteins, suggesting a potential evolutionary relationship.
Suggests shared antigens for cross-protection strategies; leaves open translation from animal models to human hMPV applications.
Avian pneumovirus subgroup C (APV/C) has recently been reported to be more closely related to human metapneumovirus (hMPV) as determined by sequence analysis. To examine the antigenic relationship between APV/C and hMPV, the APV/C fusion (F) gene was cloned and expressed as an uncleaved glycoprotein in a baculovirus system. The reactivity of the APV/C F protein with antibodies against APV subgroups A, B, C, and hMPV was examined by Western blot analysis. The results showed that the expressed APV/C F protein was not only recognized by APV/C-specific antibodies but also by antibodies raised against hMPV. Previously expressed recombinant hMPV F protein also reacted with APV/C-specific antibodies, suggesting that there was significant antigenic cross-reactivity and a potential evolutionary relationship between hMPV and APV/C. Interestingly, the recombinant F proteins from APV/C and hMPV were not recognized by polyclonal antibodies specific to APV subgroups A and B.
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Luo et al. (2009) studied Avian pneumovirus and human metapneumovirus. Recombinant fusion proteins was evaluated on Antigenic cross-reactivity. Recombinant APV/C fusion protein was recognized by both APV/C-specific and hMPV-specific antibodies, demonstrating significant antigenic cross-reactivity between the two viruses.
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