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August 1, 1991Molecular EndocrinologyOpen Access

Insulin Rapidly Inhibits Insulin-Like Growth Factor-Binding Protein-1 Gene Expression in H4-II-E Rat Hepatoma Cells

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Population

H4-II-E cell line derived from the well differentiated H35 rat hepatoma

Comparison

Insulin incubation for up to 24 hours vs Control conditions

Design

Preclinical

Follow-up

up to 24 hours

Authors

COCraig C. OrlowskiUniversity of Rochester Medical CenterGOGuck T. OoiUniversity of California, RiversideDBDaniel R. BrownUniversity of Florida

Discussion

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Implication

Should not change clinical practice; leaves open human relevance of insulin's transcriptional effects on IGFBP-1.

Structured PICO

P
Population
H4-II-E cell line derived from the well differentiated H35 rat hepatoma
I
Intervention
Insulin incubation (physiological concentrations, ED50 < 0.5 nM) for up to 24 hours
C
Comparator
Control conditions (absence of insulin, or presence of dexamethasone)
O
Outcome
IGFBP-1 mRNA abundance and gene transcription ratesurrogate

Insulin rapidly and directly inhibits IGFBP-1 gene transcription in rat hepatoma cells, providing a molecular basis for its in vivo regulation.

Cite This Study

Orlowski et al. (1991) studied this question.

synapsesocial.com/papers/6a8851729a71e8b12ff4dcdbhttps://doi.org/10.1210/mend-5-8-1180
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Enhanced Expression of Insulin-Like Growth Factor-Binding Protein-I in the Fasted Rat: The Effects of Insulin and Growth Hormone Administration*1991 · 64 citations
  2. 2Insulin-Like Growth Factor I (IGF-I)-Binding Protein Complex Is a Better Mitogen than Free IGF-I*1989 · 314 citations
  3. 3The Nature and Regulation of the Receptors for Insulin-Like Growth Factors1985 · 38 citations
  4. 4Acute metabolic effects and half-lives of intravenously administered insulinlike growth factors I and II in normal and hypophysectomized rats.1986 · 304 citations
  5. 5Cultured Hepatoma Cells as a Model System for Studying Insulin Processing and Biologic Responsiveness1980 · 83 citations