Spawning sockeye salmon from the Wauk Wash River, B.C., contained 65% less carotenoid than prespawning fish taken at sea near Juneau, Alaska. Free astaxanthin in the muscle of spawning fish was only 1% of the amount noted for prespawning fish. Ninety-five percent of the pigment retained by the spawning males was located in the skin as esters and 85% of the pigment of spawning females was deposited in the eggs as free astaxanthin.
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George F. Crozier (1970) studied this question.
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