Indocyanine Green-001 (ICG-001) Attenuates Wnt/β-catenininduces Myocardial Injury Following Sepsis

Figures

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  Figure 1. The mean of plasma proinflammatory cytokines (ng/ml) in the four experimental groups 24 h after cecal ligation and puncture. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham, **P < 0.05 versus untreated and vehicle.

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  Figure 2. The mean of myocardial tissue proinflammatory cytokines (pg/mg) in the four experimental groups 24 h after cecal ligation and puncture. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 3. The mean of plasma monocyte chemoattractant protein-1 (ng/ml) in the four experimental groups 24 h after cecal ligation and puncture. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 4. The mean of myocardial tissue monocyte chemoattractant protein-1 (pg/mg) in the four experimental groups 24 h after cecal ligation and puncture. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 5. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 6. The mean of relative Wnt activity in the four experimental groups 24 h after cecal ligation and puncture. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 7. The mean of relative β-catenin activity analysis by Western blot in the four experimental groups 24 h after cecal ligation and puncture. Glyceraldehyde-3-phosphate dehydrogenase was used to normalize the Western blots. Data are expressed as mean ± standard error, n = 8 in each group; *P < 0.05 versus corresponding sham; **P < 0.05 versus untreated and vehicle

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  Figure 8. Indocyanine green treatment improved histopathological changes after sepsis. Heart tissue from cecal ligation and puncture demonstrating extensive contraction band change (black arrows) and extensive extravasation of red blood cells (white arrowhead) with margination of polymorphonuclear leukocytes (white arrows) and interstitial edema (black arrowhead [A - sham, B - cecal ligation and puncture, C - Vehicle, D - indocyanine green-001])

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  Figure 9. Cardiac damage score. Data are expressed as mean±standard error, n=8 in each group; *P<0.05 versus corresponding sham; **P<0.05 versus untreated and vehicle