On-capillary raman spectroscopy of nucleoside/borate complexes separated by isotachophoresis

On-capillary normal Raman spectroscopy with 532 nm excitation is used as a detection method for capillary isotachophoresis (ITP) of the nucleosides: adenosine, cytidine, guanosine, and uridine. The compounds are separated as their borate complexes between leading electrolyte 0.1 M Cl−, pH 7.5, and terminating electrolyte 0.1 M B(OH)4−, pH 10. The Raman spectra of the nucleoside/borate complexes are presented. Additionally, Raman spectra are obtained from separated adenosine and adenosine 5′-triphosphate (ATP). Starting concentrations in the range 5.0×10−6–5×10−4 M are employed. Preconcentration factors of at least 103 are observed.