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Prior studies have established a definite interrelationship between the salivary glands and the endocrine system. This study was designed to histochemically characterize testosterone‐induced changes in the submandibular and the sublingual glands of male and female Ajax strain mice. In addition to morphologic stains, histochemical procedures included the demonstration of tryptophan, tyrosine, and sulfhydryl groups. Alcian blue, PAS, and toluidine blue staining was also employed. The Gomori and azo dye coupling procedures were both utilized for the demonstration of alkaline phosphatase.
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Kronman et al. (1965) studied this question.
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