The metabolism of isolated rat-liver nucleoli and other subnuclear fractions. The active site of amino acid incorporation in the nucleus

It was shown by Rees & Rowland (1961) that rat-liver nuclei isolated in 025 M-sucrose incorpor- ated amino acids into protein, and nucleotides into RNA. Although the incorporation was inhibited to varying degrees by anoxia and by several inhibitors of oxidative phosphorylation, it was not affected by detergents, by freezing and thawing the nuclei, or