Directed evolution. An orthogonal amber suppressor tRNA/aminoacyl–tRNA synthetase pair was evolved that allows the site-specific incorporation of 4′-[3-(trifluoromethyl)-3H-diazirin-3-yl]-L-phenylalanine into proteins in Escherichia coli with high efficiency and fidelity.
No takes yet. Share an insight, caveat, or question.
Tippmann et al. (2007) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: