Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.
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Osamura et al. (2000) studied this question.
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