Phase dependent phase shifts induced by pyruvate and acetaldehyde in oscillating NADH of yeast cells

NADH oscillations of glycolysing yeast cells are phase shifted by the addition of acetaldehyde or pyruvate in low concentrations (0.05 resp. 0.07 mM) depending on the original phase of NADH at which these chemicals are added. Phase shift is most pronounced at the inflection point of rising NADH, it is medium if NADH is at its maximum or minimum and the effect becomes negligible at the inflection point of falling NADH.