The effects and possible mechanism of β2AR gene expression in cardiocytes of canines with heart failure

Figures

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  Figure 1.. Four chambers of the heart before and after pacing. (A) Before pacing. (B) Pacing for 4 weeks.

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  Figure 2.. β2AR protein expression of cardiocytes in control group and heart failure group after transfection with EGFP or β2AR-EGFP for 48 h. Con vs. con + β2, HF vs. HF + β2, P<0.05, n=4. β2AR, β2-adrenergic receptor; EGFP, enhanced green fluorescent protein; con, control; HF, heart failure.

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  Figure 3.. Changes of intracellular cAMP concentration after transfection with β2AR-EGFP. (A and B) Compared with con, *P<0.05, n=6; compared with con-ISO, **P<0.01, n=6. (C) Compared with HF, *P<0.05, n=6; compared with HF-ISO, **P<0.01, n=6. β2AR, β2-adrenergic receptor; EGFP, enhanced green fluorescent protein; con, control; HF, heart failure; ISO, isoproterenol.

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  Figure 4.. Contractile function of cardiocytes in canines separated immediately. (A) Compared with control group, *P<0.05 in basic state; **P<0.01 in the state of maximal contraction. (B) compared with control group, *P<0.05 in basic state. (C) Compared with control group, *P<0.05 in the state of maximal contraction. Con, control; HF, heart failure.

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  Figure 5.. Contractile function of control cardiocytes after culture for 48 h. (A) Compared with control group, *P<0.05 in basic state. (B) Compared with control group, *P<0.01 in basic state; **P<0.01 in the state of maximal contraction. (C) Compared with control group, *P<0.05 in the state of maximal contraction. EGFP, enhanced green fluorescent protein; con, control.

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  Figure 6.. Ca2+ concentration and semi-logarithmic cumulative concentration of ISO-the percentage of contraction amplitude curve of cardiocytes in control group canines after cultured for 48 h.

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  Figure 7.. Contractile function of cardiocytes in heart failure (HF) group canines after cultured for 48 h. (A) Compared with control group, *P<0.05 in basic state; **P<0.01 in the state of maximal contraction. (B) Compared with control group, *P<0.05 in basic state; **P<0.01 in the state of maximal contraction. (C) R50 of cardiocytes in the HF group did not change significantly in either the basic or maximal contraction state after transfection with β2AR-EGFP.

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  Figure 8.. Ca2+ concentration and semi-logarithmic cumulative concentration of isoproterenol (ISO)-the percentage of contraction amplitude curve of cardiocytes in heart failure (HF) group canines cultured for 48 h.