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July 7, 2007Applied and Environmental MicrobiologyOpen Access

Development and Preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples

Panagiotis Karanis Oriel Thekisoe Klytaimnistra Kiouptsi Jerry E. Ongerth Ikuo Igarashi Noborou Inoue

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Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

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Karanis et al. (2007) studied this question.

synapsesocial.com/papers/6a8909adcd9902a7ca126ea9 https://doi.org/10.1128/aem.01152-07

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