DbcAMP regulates adipogenesis in sheep inguinal preadipocytes

Figures

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  Fig. 1. Effect of the dose of dbcAMP on the proliferation of sheep preadipocytes on Day1, Day2, Day3, Day4, Day5 and Day6. Sheep preadipocytes were grown in proliferation medium containing 0 nmol/l (as a control), 1 nmol/l, 10 nmol/l, 1 × 102 nmol/l, 1 × 103 nmol/l, 1 × 104 nmol/l, 1 × 105 nmol/l, or 1 × 106 nmol/l dbcAMP for 6 days and the cell proliferation was determined by MTT Cell Proliferation Assay everyday of the 6 days. Statistical differences reported are among doses. Values are means ± SEM; n = 4. Values with different letters are significantly different (P < 0.05)

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  Fig. 2. Effect of the dose of dbcAMP on the differentiation of sheep preadipocytes on Day2, Day4, Day6, Day8, Day10 and Day12. After cell differentiation induction, sheep preadipocytes were grown in proliferation medium containing 0 nmol/l (as a control), 1 nmol/l, 10 nmol/l, 1 × 102 nmol/l, 1 × 103 nmol/l, 1 × 104 nmol/l, 1 × 105 nmol/l, or 1 × 106 nmol/l dbcAMP for 12 days and the cell differentiation was determined by Oil Red O Cell Differentiation Assay every two days of the 12 days. Statistical differences reported are among doses. Values are means ± SEM; n = 4. Values with different letters are significantly different (P < 0.05)

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  Fig. 3. Effect of the dose of dbcAMP on the expression of SCD mRNA (a) and HSL mRNA (b) in sheep preadipocyte cultures. Sheep preadipocytes from inguinal adipose tissue were grown in proliferation medium containing 0 nmol/L (as a control), 1 nmol/l, 1 × 102 nmol/l, 1 × 104 nmol/l or 1 × 106 nmol/l dbcAMP for 4 days after cell differentiation induction. Total RNA was harvested and used for first-strand cDNA synthesis. Quantitative real-time PCR analyses were performed to analyze the expression of SCD mRNA and HSL mRNA. The relative expression abundance of a given gene was calculated after normalization to GAPDH mRNA expression. Statistical differences reported are among doses. Values are means ± SEM; n = 3. Values with different letters are significantly different (P < 0.05)

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  Fig. 4. Effect of the dose of dbcAMP on the synthesis of various fatty acids in sheep preadipocyte cultures. Sheep preadipocytes were grown in proliferation medium containing 0 nmol/l (a vehicle control), 1 nmol/l, 1 × 102 nmol/l, 1 × 104 nmol/l or 1 × 106 nmol/l dbcAMP for 4 days after cell differentiation induction. After various fatty acids of sheep preadipocytes were extracted, fatty acid methyl esters were analyzed by gas chromatography method. Only C20:0 at the different levels of dbcAMP had significant difference. Statistical differences reported are among doses. Values are means ± SEM; n = 3. Values with different letters are significantly different (P < 0.05)