Organ culture of rat mesenteric arteries induces a PKC-dependent transcriptional up-regulation of 5-HT2A receptors and down-regulation of AT1 receptors, altering vascular contractility.
Organ culture may confound ex vivo vascular reactivity assays; leaves open relevance to human disease.
The purpose of this study was to investigate in rat mesenteric artery if there is up-regulation of 5-hydroxytryptamine (5-HT) receptors and angiotensin II receptors and the potential role of protein kinase C activation in the smooth muscle cells during organ culture. Angiotensin II, 5-HT and potassium induced contraction of ring segments without endothelium, monitored by a sensitive in vitro pharmacology method. After the culture of the arterial ring segments for 24 hr, the concentration-contraction curves induced by 5-HT slightly shifted towards to the left with pEC(50) from 6.64+/-0.11 to 6.84+/-0.11 and a significant increase in E(max) from 147+/-11% to 246+/-15% (P<0.05), compared with that obtained in fresh segments. In contrast, the angiotensin II concentration-contraction curve only showed a significant decrease in E(max) from 99+/-10% to 37+/-8%. Specific antagonists for the 5-HT type 2A receptors (5-HT(2A)) and angiotensin II type 1 receptors (AT(1)) demonstrated that the contractions occurred via 5-HT(2A) and AT(1) receptors, respectively. Real-time PCR revealed that the 5-HT(2A) receptor mRNA was up-regulated in parallel with the contractile response while there was a down-regulation of AT(1) receptor mRNA. Transcriptional inhibitor actinomycin D and specific protein kinase C inhibitor Ro31-8220 demonstrated that it was a transcriptional mechanism with involvement of protein kinase C that regulated the enhanced expression of 5-HT(2A) receptors in the mesenteric artery.
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Luo et al. (2004) studied this question.
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