Lee and Burris (1943) obtained sufficient azotobacter cells, using a 200-gallon fermentor, to demonstrate the presence of several enzymes in cell-free preparations.Research of the past decade has increased considerably our knowledge of how to prepare bacterial enzymes and how specific enzymes vary with experimental condi- tions and with physiological age of the organism.It is evident that enzymes of these earlier preparations from physiologically old cells possessed low specific activi-
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MacLeod et al. (1954) studied this question.
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