Ion‐pair and ion‐suppression mode high performance liquid chromatography was conducted on the alkaloidal constituents of the roots of Artabotrys monteiroae, an important medicinal plant in Kenya having analgesic and anti‐inflammatory action. Use of sodium perchlorate as an ion‐pair reagent led to excellent separation. In the ion‐suppression system containing ammonium acetate, the separation was rather poor, but liquid chromatography‐mass spectrometry was possible, which was not the case in the ion‐pair system. Four aporphines, (‐)‐asimilobine, (+)‐wilsonirine, (+)‐norisoboldine and anonaine, and a benzylisoquinoline, (+)‐reticuline, were identified after isolation by preparative thin layer chromatography.
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Kato et al. (1993) studied this question.