The molecular weights of growth hormone and prolactin from 5 species were determined by disc electrophoresis. Human, bovine, ovine and murine (rat and mouse) hormones were studied. The method permitted simultaneous evaluation of the molecular weight of the principal component, the aggregated forms and the deamidated modifications. For ovine, bovine and murine growth hormones, an extrapolation to infinite dilution was required to obtain a minimum molecular weight. Concentration-dependent association effects were eliminated if the maleylated derivatives were used. This assured a minimum molecular weight but the multiple components could not be distinguished. Human growth hormone and the prolactins did not exhibit the association phenomenon during electrophoresis. The molecular weights in general agreed quite well with reported values but the method tended to give values that were slightly higher than those found by ultracentrifugation, especially in the case of bovine growth hormone. (Endocrinology85: 465, 1969)
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CHEEVER et al. (1969) studied this question.