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Develop a radioimmunoassay utilizing purified human liver ligandin to quantify serum ligandin levels across healthy controls and liver disease patients.

January 1, 1977KanzoOpen Access

Quantification of human serum ligandin by radioimmunoassay

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Develop a radioimmunoassay utilizing purified human liver ligandin to quantify serum ligandin levels across healthy controls and liver disease patients.
Purified ligandin from human liver tissue to establish a sensitive radioimmunoassay with a detection threshold of 1 ng/ml.
Quantified serum ligandin levels in healthy individuals and patients with acute hepatitis (n=8), chronic hepatitis (n=12), liver cirrhosis (n=29), and constitutional jaundice (n=5).
Normal serum ligandin was 3.7 ± 1.1 ng/ml (maximum 5.3 ng/ml), compared to elevated levels in acute hepatitis (16.8 ± 11.1 ng/ml), chronic hepatitis (36.3 ± 19.0 ng/ml), and cirrhosis (19.6 ± 15.1 ng/ml).
Ligandin concentrations correlated with serum GPT levels in acute and chronic hepatitis, but not in liver cirrhosis, while constitutional jaundice cases showed near-normal levels.

Implication

Assay validation study reveals elevated serum ligandin across various liver diseases, indicating its utility as a biomarker for active hepatic damage.

Abstract

ヒト肝臓より抽出精製したリガンディンを用いてradioimmunoassay(以下RIA)法を開発し,血清リガンディンの定量を行った. 測定法の感度は1ng/mlで再現性は良好であり,血清による非特異的影響は認められなかった. 血清リガンディンの正常値は3.7±1.1(Mean±S.D.)ng/mlであり,最高値は5.3ng/mlであった.急性肝炎8例では16.8±11.1,慢性肝炎12例では36.3±19.0,肝硬変症29例では19.6±15.1であり,疾患の増悪期に高値を呈するものを多く認あた.体質性黄疸5例では,ほぼ正常に近い値を示した. 急性肝炎および慢性肝炎では,血清GPT値との相関を認めたが,肝硬変症では相関を認めなかった.

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Cite This Study

TSURU et al. (1977) studied this question.

synapsesocial.com/papers/6a893bfdb4e829b19bd43a01 https://doi.org/10.2957/kanzo.18.919

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Implication

Assay validation study reveals elevated serum ligandin across various liver diseases, indicating its utility as a biomarker for active hepatic damage.

Quantification of human serum ligandin by radioimmunoassay

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Implication

Abstract

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Cite This Study

Quantification of human serum ligandin by radioimmunoassay

Key Points

Implication

Abstract

Discussion

Cite This Study

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