Why the study?
Human Coxsackievirus B5 is associated with myocarditis, meningitis, and other diseases and has important public health impact, warranting rapid and specific molecular detection methods.
A newly developed SYBR Green-based real-time PCR assay provides a rapid and specific method for detecting Human Coxsackievirus B5, which is relevant for diagnosing viral myocarditis and other associated diseases.
SYBR Green PCR offers a viable HuCoxV-B5 detection alternative; extends TaqMan methods but leaves clinical translation open.
Human Coxsackievirus B5 (HuCoxV-B5) infection has been associated with various diseases such as myocarditis, aseptic meningitis, hand-foot-and mouth-disease, and insulin-dependent diabetes. HuCoxV-B5 is a virus transmitted through the fecal-oral route and is detected in clinics, aquatic environments, food, shellfish, etc. and is one of the more important viruses in public health because of its incidence rate reported worldwide. In this study, a combination of SYBR Green-based real-time PCR primers for molecular diagnosis including monitoring of HuCoxV-B5 was selected and the optimal reaction conditions were established. Compared with the previously reported TaqMan probe-based real-time PCR method, assessments including a sample applicability test were performed. Results showed that the real-time PCR method developed in this study was suitable for a molecular diagnostic technique for detecting HuCoxV-B5. This study is expected to contribute to efforts in responding to safety accidents in public health because the proposed method facilitates rapid diagnosis of clinical patients. It can also be used as a specific monitoring tool of HuCoxV-B5 in non-clinical areas such as aquatic environments among others.
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Kyu Bong Cho (2020) studied this question.
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