Functional Antibody Arrays through Metal Ion‐Affinity Templates

Immobile but active. Unmodified antibodies can be immobilized in an active state on arrays of patterned features coupled with metal-ions by using dip-pen nanolithography and microcontact printing. Their immunoreactivity was determined by atomic force and fluorescence microscopy after the arrays had been exposed to their corresponding protein or virus antigens. An example of a functional antibody array used to capture the influenza virus is shown in the AFM image.