Primary structure of a chloramphenicol acetyltransferase: Mass spectrometric studies

Low resolution electron impact mass spectrometry has been used as a major tool in the sequencing of a chloramphenicol acetyltransferase (mol. wt 25 668). Mass spectrometric data from mixtures of peptides from elastase, chymotryptic and subtilisin digests have defined 83% of the protein sequence. Several, previously unrecognized, peptide fragmentation pathways are reported.