Answers are grounded in this paper and cite their sources.
A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.
No takes yet. Share an insight, caveat, or question.
Gubala et al. (2006) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: