Experimental platform demonstrates rapid isolation of stable antibody-producing CHO cell lines using droplet microfluidics, suggesting a streamlined workflow for biomanufacturing.
Key Points
To develop a high-throughput droplet microfluidic workflow utilizing a FRET-based secretion assay and image-verified monoclonality to accelerate stable CHO cell line development.
Optimized a picodroplet-based FRET secretion assay by evaluating probe configurations, donor-to-acceptor ratios, and signal saturation ranges.
Integrated microfluidic droplet screening with sequential cell enrichment and image-based single-cell isolation in ExpiCHO-S cells.
Generated image-verified monoclonal lines that maintained consistent antibody production during stability testing, including without selection pressure.
Reduced the early clone-identification timeline from DNA transfection to validated 96-well antibody-producing clones to approximately 5 weeks.