Protocol for untargeted multi-omics dried blood spot extraction using liquid chromatography-tandem mass spectrometry

Dried blood spots (DBS), most notably employed in newborn screening, present a valuable opportunity to analyze whole blood via a stable and simplified sample collection. DBS are generally used to evaluate one class of compounds at a time. Herein, we present a protocol to semi-quantitatively evaluate metabolites and lipids from the same DBS using a multi-omics approach. We describe steps for extracting both metabolites and lipids from the paper-based sample and analyzing them via liquid chromatography-tandem mass spectrometry. For complete details on the use and execution of this protocol, please refer to Li et al. 1