Protocol demonstrates untargeted polar metabolite profiling using anion-exchange chromatography and mass spectrometry, highlighting improved detection of cellular phenotypes.
Polar metabolites are challenging to analyze using common reverse-phase chromatography. However, these molecules are most essential for deciphering biological phenotypes. Here, we present a protocol for polar metabolite analysis based on anion-exchange chromatography coupled with high-resolution mass spectrometry (AEC-HRMS). We outline preparation techniques for common biological matrices, instrumental setup for untargeted metabolomics, including sample analysis and essential data treatment.
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Jouan et al. (2026) studied this question.
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