A tricomponent sensor was designed for genotyping stem-loop-structured DNA sequences. DNA adaptor strand f (see picture; red) hybridizes to the analyte (orange) with high affinity and unwinds its secondary structure; strand m (green) forms a stable complex only with the fully complementary analyte sequence. A fluorescing molecular beacon reports complex formation.
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Grimes et al. (2010) studied this question.
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