February 1, 1992Journal of Clinical MicrobiologyOpen Access

In vitro transcription and translation of group B rotavirus strain IDIR gene 8 and immunoprecipitation by human sera

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Population

129 human serum specimens from healthy adult males in Cincinnati, Ohio, and adults in Boston, Mass…

Design

Preclinical

Authors

JEJoseph J. EidenPfizer (United States)SWS B WeeUniversity of North Carolina at Chapel Hill SVSteven VonderfechtUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Anti-IDIR antibodies detectable in some U.S. sera; hypothesis-generating and should not yet inform clinical practice.

Structured PICO

Population

129 human serum specimens from healthy adult males in Cincinnati, Ohio, and adults in Boston, Mass., Baltimore, and Bethesda, Md., as well as animal sera (rat, guinea pig, rabbit).

Intervention

In vitro transcription and translation of group B rotavirus strain IDIR gene 8 to produce a specific polypeptide antigen.

Outcome

Specific immunoprecipitation of the IDIR gene 8 polypeptide by human sera.

The IDIR gene 8 polypeptide can be synthesized in vitro and specifically immunoprecipitated by a small percentage of human sera, confirming the presence of anti-IDIR antibodies in the U.S. population.

Limitations

In vitro transcription and translation did not permit synthesis of the polypeptide in quantities sufficient for enzyme-linked immunosorbent assay format.
The immunoprecipitation format did not afford the greatest sensitivity.

Cite This Study

Eiden et al. (1992) studied this question.

synapsesocial.com/papers/6a89702b991508d05dc9851f https://doi.org/10.1128/jcm.30.2.440-443.1992

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In vitro transcription and translation of group B rotavirus strain IDIR gene 8 and immunoprecipitation by human sera

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

In vitro transcription and translation of group B rotavirus strain IDIR gene 8 and immunoprecipitation by human sera

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider