Filamin A phosphorylation by Akt promotes cell migration in response to arsenic

// Lingzhi Li 1 , Yongju Lu 1 , Paul M. Stemmer 1, 2 , Fei Chen 1 1 Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA 2 The Proteomics Core and Institute of Environmental Health Sciences, School of Medicine, Wayne State University, Detroit, MI 48201, USA Correspondence to: Fei Chen, e-mail: fchen@wayne.edu Keywords: arsenic, Akt, filamin A, migration, patient survival Received: February 12, 2015 Accepted: March 14, 2015 Published: April 03, 2015 ABSTRACT We had previously reported that trivalent arsenic (As 3+ ), a well-known environmental carcinogen, induces phosphorylation of several putative Akt substrates. In the present report, we characterized one of these substrates by immunoprecipitation and proteomics analysis. The results indicate that a cytoskeleton remodeling protein, filamin A, with a molecular weight around 280 kDa, is phosphorylated by Akt in HEK-293 cells treated with As 3+ , which was also confirmed in human bronchial epithelial cell line, BEAS-2B cells. Additional biochemical and biological studies revealed that serine 2152 (S2152) of filamin A is phosphorylated by activated Akt in the cells treated with As 3+ . To further confirm the importance of Akt-dependent filamin A S2152 phosphorylation in As 3+ -induced cell migration, we over-expressed either wild type filamin A or the mutated filamin A in which the S2152 was substituted with alanine (S2152A). The capability of cell migration was reduced significantly in the cells expressing the mutated filamin A (S2152A). Clinically, we found that increased expression of filamin A predicts poorer overall survival of the lung cancer patients with adenocarcinoma. Thus, these data suggest that Akt dependent filamin A phosphorylation is one of the key events in mediating As 3+ -induced carcinogenesis. Antagonizing Akt signaling can ameliorate As 3+ -induced filamin A phosphorylation and cell migration, which may serve as a molecular targeting strategy for malignancies associated with environmental As 3+ exposure.