A feeding experiment was conducted to investigate the effect of Bacillus subtilis bacterium, on larval growth and development rate of Macrobrachium rosenbergii (de Man) during February 28 to April 8, 2005 in University Putra Malaysia hatchery. Newly hatched larvae of M. rosenbergii were reared with two dietary treatments consisting of newly hatched Artemia salina nauplii with B. subtilis (108 cells ml−1), and newly hatched A. salina nauplii without B. subtilis carried out in triplicate in 60-L aquarium (50 L−1). After trial, the larvae that fed B. subtilis-treated Artemia naupli were found to have higher survival and a faster rate of metamorphosis than larvae that were fed with nontreated Artemia naupli. There were significant differences between B. subtilis-treated Artemia naupli and nontreated Artemia diet in larval growth and development rate of metamorphosis (P < 0.05). Larval survival after 40 days was significantly greater (P < 0.05) in the B. subtilis-treated groups (55.3 ± 1.02) compared with the nontreated groups (36.2 ± 5.02%).
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Keysami et al. (2007) studied this question.
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