A method for the determination of metribuzin and its metabolites in plant tissues has been developed using High Pressure Liquid Chromatography (HPLC). The system used involves reversed-phase chromatography on a C-18 HPLC column and a 62:38 methanol/0.05 M acetic acid mobile phase. Under these conditions, metribuzin and the three known metabolites [deaminated metribuzin (DA), deaminated diketometribuzin (DADK) and diketometribuzin (DK)] are completely resolved from each other. Detection is by UV absorbance at 254 nm.
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Parker et al. (1983) studied this question.
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