GMRSP encoded by lncRNA H19 regulates metabolic reprogramming and alleviates aortic dissection

Fig. 1. Downregulation of GMRSP expression in human aortic dissection. A Heatmap of the top 15 differentially expressed lncRNAs in normal human aortic tissues and AD tissues (q < 0.05, |Log2FC | >1). B Q-PCR analysis of H19 levels in the aorta of controls without AD (n = 7 biologically independent samples) and patients with AD (n = 9 biologically independent samples). C The 1253-nucleotide lncRNA H19 contains a potential small protein-encoding ORF, which may encode a 131-amino acid small protein, GMRSP. D Western blot analysis of the levels of GMRSP in the aorta of controls without AD (n = 7 biologically independent samples) and patients with AD (n = 9 biologically independent samples). E Representative images of GMRSP (red) co-localized with α-SMA (green)-positive vascular smooth muscle cells in aortic tissues from controls without AD (n = 5 biologically independent samples) and patients with AD (n = 5 biologically independent samples), Scale bar: 100 μm. After transfecting the indicated constructs into primary human VSMCs cells for 48 h, GFP fluorescence was detected by fluorescence microscope, Scale bar: 50 μm (F), and GMRSP-GFP fusion protein expression was detected by western…

Fig. 2. GMRSP overexpression represses thoracic aortic dissection formation. A Male mice were induced with tamoxifen for 5 days at two weeks. One week after the last tamoxifen treatment, GMRSPOE-flox and SM22α+GMRSPOE-flox mice were treated with 0.25% BAPN monofumarate for four weeks (n = 10 biological replicates per group). B Representative images of the aorta of the mice are shown. C The survival rate was estimated by the Kaplan-Meier method and compared using the Breslow test (n = 10 biological replicates per group). D The incidence of AD was statistically analyzed. E The maximum aortic diameter was measured (n = 10 biological replicates per group). F Representative macroscopic images of aorta sections stained with H&E and EVG, Scale bar: 200 μm and 50 μm. The wall thickness (G) and elastin integrity (H) of each groups were analyzed (n = 10 biological replicates per group). I Immunofluorescence staining of α-SMA (green) and GMRSP (red) in the aorta of the mice is shown. Nuclei were counterstained with DAPI. J Immunofluorescence staining of SM22α (green) and MMP2 (red) in the aorta of the mice is shown. Nuclei were counterstained with DAPI,Scale bar: 200 μm. Data are presented as the…

Fig. 3. GMRSP is required for maintaining oxidative metabolism via regulating the PKM pre-mRNA splicing in VSMCs. A Dotplot showing metabolic characteristics of vascular smooth muscle cells from patients with/without aortic dissection using scMetabolism algorithm based on the GSE213740. The circle size and color darkness both represent the scaled metabolic score. B, C GO analysis (D) and KEGG (E) analysis between H19-positive and H19-negative vascular smooth muscle cells in GSE213740. D After transfection with the indicated constructs for 36 hours, followed by PDGF-BB (20 ng/mL) treatment for 24 h, RNA sequencing was performed in human primary VSMCs. GMRSP regulates PKM pre-mRNA splicing, promoting PKM1 isoform formation and inhibiting PKM2 isoform formation. E Representative immunofluorescent images of F-actin (red), PKM1 (yellow), and PKM2 (green) in human primary VSMCs were determined, Scale bar: 50 μm. The seahorse glycolysis stress test (n = 8 biologically independent samples) (F) and representative images of TMRE staining, Scale bar: 50 μm (G), F-actin and MitoTracker staining, Scale bar: 5 μm (H) were obtained in human primary VSMCs transfected with the indicated constructs for 36…

Fig. 4. Activating PKM2 reverses the protective effect of GMRSP in both in vitro and in vivo. Seahorse glycolysis stress test (n = 8 biologically independent samples) (A), representative images of TMRE staining, Scale bar: 50 μm (B), F-actin and MitoTracker staining, Scale bar: 50 μm (C) were performed in human primary VSMCs transfected with the indicated constructs for 36 h, followed by TEPP-46 (20 µmol/L) and PDGF-BB (20 ng/mL) treatment for 24 h. D SM22α+GMRSPOE-flox mice were induced with tamoxifen for 5 days at two weeks. One week after the last tamoxifen treatment, mice were treated with 0.25% BAPN monofumarate and TEPP-46 (10 mg/kg) for four weeks (n = 10 biological replicates per group). E Representative images of the mouse aorta are shown. F Maximum aortic diameter measurement (n = 10 biological replicates per group). G Survival rate estimation using the Kaplan-Meier method and comparison using the Breslow test (n = 10 biological replicates per group). H Representative macroscopic images of aorta sections were stained with H&E and EVG, Scale bar: 200 μm and 50 μm. The wall thickness (I) and elastin integrity (J) of each groups were analyzed(n = 10 biological replicates per…

Fig. 5. The role of the expression level of PKM2 in plasma in the diagnosis and prognosis of AD. A The PKM splicing assay was performed in the aorta of controls without AD (n = 7 biologically independent samples) and patients with AD (n = 9 biologically independent samples). B The levels of PKM1 and PKM2 mRNA were positively and negatively correlated with the levels of GMRSP mRNA in the aorta of controls without AD (n = 7 biologically independent samples) and patients with AD (n = 9 biologically independent samples), respectively. C Immunofluorescence staining of PKM1, GMRSP, PKM2 and SM22α in the aorta from controls without AD (n = 5 biologically independent samples) and patients with AD (n = 5 biologically independent samples) were shown. Nuclei were counterstained with DAPI, Scale bar: 100 μm. D Distribution of plasma levels of PKM2 in healthy controls (n = 60 biologically independent samples) and patients with type B AD (n = 89 biologically independent samples)or type A AD (n = 56 biologically independent samples). E Receiver operating characteristic curves: patients with AD vs. healthy controls for PKM2. F The discrepancy of the maximum aortic diameter in patients with high (>2.40…

Fig. 6. GMRSP Interacts with the Gly-rich domains of hnRNP A2B1. A Silver staining identifies the specific bands for GMRSP-co-IP. B The GMRSP-Flag plasmid was transfected into HEK293T cells, cellular lysates were treated with 10 mg/mL RNase A for one hour or left untreated, GMRSP-Flag complexes were co-IPed by anti-Flag antibody, and hnRNPA2B1 was determined. C The hnRNPA2B1-HA plasmid was transfected into HEK293T cells, cellular lysates were treated with 10 mg/mL RNase A for one hour or left untreated, hnRNPA2B1-HA complexes were co-IPed by anti-HA antibody, and GMRSP was determined. D Immunofluorescence of GMRSP (red) co-localized with hnRNPA2B1 (green) in VSMCs. Nuclei were stained with DAPI (blue), Scale bar: 20 μm. E An illustration of the truncated hnRNPA2B1 domains is shown. F, G The WT and the indicated mutants of hnRNPA2B1-HA, together with the GMRSP-Flag vector, were co-transfected into HEK293T cells. hnRNPA2B1-HA (F) and GMRSP-Flag (G) complexes were co-IPed with anti-HA and anti-Flag antibodies, respectively, to detect GMRSP-Flag and hnRNPA2B1-HA, respectively. H Molecular docking analysis of the GMRSP and hnRNPA2B1 Gly-rich domains. I, L The indicated hnRNPA2B1-HA mutant…

Fig. 7. GMRSP Blocks the Increase of PKM2, the Decrease of PKM1, glycolysis, and mitochondrial dysfunction Induced by hnRNP A2B1 overexpression. A–F The GMRSP-Flag vector, together with the hnRNPA2B1-HA vector, was co-transfected into human primary VSMCs for 36 h, followed by PDGF-BB (20 ng/mL) treatment for 24 h (n = 5 per group). The indicated protein levels were detected (A). The PKM splicing assay was performed (B). Representative immunofluorescent images of F-actin (red), PKM1 (yellow), and PKM2 (green) were determined, Scale bar: 50 μm (C). The seahorse glycolysis stress test (n = 8 biologically independent samples) (D) representative images of TMRE staining, Scale bar: 50 μm (E), and MitoTracker staining, Scale bar: 50 μm (F) were performed. Data are presented as the mean ± SD. The p values were calculated by a two-tailed unpaired Student’s t-test in D. The data presented in (A–F) are representative of three independent experiments. Source data are provided as a Source Data file.

Fig. 8. GMRSP-EVs improve AD formation in vivo. A Representative images of EVs were visualized using TEM. Scale bars: left, 50 nm; right, 100 nm. B The particle size of EVs was measured by NTA. C Representative images of the internalization of mCherry (red)-labeled EVs were visualized, Scale bar: 50 μm. D A schematic diagram of the experimental therapy: mice were intravenously injected with 500 ug/kg/day EVs for 7 days. Meanwhile, EV-treated mice were also treated with 0.25% BAPN monofumarate for four weeks (n = 15 biological replicates per group). E Representative images of the aorta of the mice are shown. F The survival rate was estimated using the Kaplan-Meier method(n = 15 biological replicates per group). G, H AD incidence and maximum aortic diameter were detected (n = 15 biological replicates per group). I Representative macroscopic images of aorta sections were performed with H&E staining and EVG staining, Scale bar: 200 μm and 50 μm. J Schematic representation of signaling events mediated by GMRSP signaling during the pathogenesis of AD.Data are presented as the mean ± SD. The p values were calculated by a two-tailed unpaired Student’s t-test in H. The p values were calculated by…