SUMMARY This investigation was initiated to separate the unsaponifiable matter of egg yolk by thin layer chromatography (TLC) and attempt to identify the steroids by gas liquid chromatography (GLC). Three GLC liquid phases, differing in selective partition properties, were used to aid in the identification of unknown steroids. A selective method was also carried out to extract the estrogenic steroids in egg yolk if present. The results of thin layer and gas chromatographic analyses indicate that a large quantity of cholesterol and considerable amounts of lanosterol, desmosterol, δ 7 ‐cholestenol, cholestanol, δ 7 ‐ and δ 8 ‐methostenol, 4a‐methyl‐δ 8,24 ‐cholestenol and its δ 7 ‐isomer, 4,4a‐dimethyl‐δ 7,24 ‐cholestenol, dihydrolanostenol, β‐sitosterol and possibly ergosterol were found to be present in egg yolk. Estrogenic steroids and other known steroid hormones were not found in egg yolk.
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Tu et al. (1970) studied this question.
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