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A collaborative study of the reagents for the radioimmunoassay of human prolactin (hPRL) to be distributed by The National Institute of Arthritis, Metabolism and Digestive Diseases was carried out. Each group applied the radioimmunoassay techniques and conditions currently used in their laboratory. The Lewis and Friesen hPRL tracers behaved identically though the former produced a more sensitive assay. Both hormones were iodinated satisfactorily by the Chloramine T method. The Friesen antiserum was more sensitive than the SLV antiserum. Similar results were obtained for each of the plasma samples supplied in the homologous and heterologous systems. The results obtained confirmed the reports of the investigators who donated the reagents.
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Aubert et al. (1974) studied this question.
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