Key result
A fluorescent reporter system using FRET allowed the rapid detection (within 7.5 h) of very low numbers (10 PFU or fewer) of infectious enteroviruses.
A novel FRET-based fluorescent reporter system enables rapid and sensitive detection of enteroviral infections.
May accelerate lab detection of enteroviruses; leaves open clinical translation from animal models.
Enteroviruses can be easily transmitted through the fecal-oral route and cause a diverse array of clinical manifestations. Recent outbreaks associated with enteroviral contamination in aquatic environments have called for the development of a more efficient and accurate virus monitoring system. To develop a simple, rapid, and direct method for identifying enteroviral infections, we generated a fluorescent reporter system in which genetically engineered cells express a hybrid fluorescent indicator composed of a linker peptide, which is exclusively cleaved by the 2A protease (2A(pro)), flanked with a cyan fluorescent protein (CFP) and a yellow fluorescent protein undergoing fluorescence resonance energy transfer. The covalent linkage between two fluorophores is disrupted due to 2A(pro) activity upon viral infection, which results in an increase in CFP intensity. This allows the rapid (within 7.5 h) detection of very low numbers (10 PFU or fewer) of infectious enteroviruses.
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Hwang et al. (2006) studied Enteroviral infection. Fluorescent reporter system using FRET was evaluated on Detection of infectious enteroviruses. A fluorescent reporter system using FRET allowed the rapid detection (within 7.5 h) of very low numbers (10 PFU or fewer) of infectious enteroviruses.
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