The purpose of this study was to investigate and correlate the effect of sensitized isologous lymphoid cells from mice with tumor-specific immunity against methylcholanthrene-induced sarcomas on the growth of these tumors in vivo and in vitro. Viable sarcoma cells were inoculated into the hind legs of isologous mice, and the legs were amputated when the tumor had grown to a diameter of about 1.0 to 1.5 em. Direct challenge with known numbers of tumor cells demonstrated specific immunity against the neoplasm used for sensitization. Lymphoid cells from these immune mice inhibited the growth of the same tumor lines when they were introduced, with the tumor cells, into irradiated isologous mice. Such a protective effect against the tumor used for immunization could be shown by passive transfer in 5 of 6 tumor lines. Aliquots of the same lymphoid cell suspensions were also tested against explants of the same tumors in tissue culture. These lymphoid cells in vitro also suppressed the tumor cells. The specificity of the inhibition of the neoplasm in vitro was demonstrated. There was a close correlation between the inhibitory effect of sensitized lymphoid cells on tumors in vivo and the suppressive effect of such lymphoid cells on these neoplasms in tissue culture.
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Rosenau et al. (1966) studied this question.