Prunetin (2) and its derivatives were synthesized in order to study the relationship between structure and attracting activity to Aphanomyces euteiches zoospore. All of the derivatives (1, 2, 4 and 6), which had a hydroxyl group at the C-5 position in the isoflavone, showed attracting activity, but the methyl ether derivatives (3, 5, 7 and 8) do not have or have very weak attracting activity. The isoflavones (1, 2 and 4) with strong attracting activity also had estrogenic activity.
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Sekizaki et al. (1988) studied this question.