Female rabbits were inseminated with semen diluted with Krebs solution at rates of 1/1000 up to 1/5000 and 0.2 cc of suspension were used. The number of spermatozoa effective for partial fertility was 16,000, and for maximum fertility was around 90,000. The group percentage of fertility resulting from vaginal insemination was slightly higher than from uterine insemination (50% vs 40%); this difference was not of statistical significance.
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Cheng et al. (1948) studied this question.