An isocratic HPLC system for quantifying benzoylecgonine (BE) and cocaine (COC) in vitreous humour (VH) was developed. Following solid-phase extraction, the two drugs are chromatographed on a column filled with octadecyl silica packing, eluted with 75% methanol in phosphate buffer of pH 7, and sensed by UV spectrophotometry at 235 and 275 nm, simultaneously. The detection limit thus achieved was 0.5 μg drug per millilitre of VH. By using tetracaine as internal standard, BE and COC can be determined with a coefficient of variation of 6 and 7%, respectively.
No takes yet. Share an insight, caveat, or question.
Fernández et al. (1994) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: