ITH great success we have been using Thayer-Martin (T-M) medium with vancomy- cin, colistimethate, and nystatin (VCN) inhibitor (1,2) for the culture of Neisseria gonorrhoeae (3).Specimens from a number of patients with purulent dis- charges containing large numbers of Gram-negative diplococci, however, have yielded no growth of gonococci on this medium.Our suspicions that some gono- cocci were suppressed by one of the three antibiotics of T-M me- dium were confirmed by the work of Reyn (4) who reported that 3 percent of gonococcal strains from 171 cultures were
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Cross et al. (1971) studied this question.
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