P-sulfonic Calix[4]Arene as Running Buffer Additive in Electrokinetic Chromatography

The application of a new water-soluble calixarene, p-sulfonic calix[4]arene, as running buffer additive in electrokinetic chromatography was first investigated. Full separation of eight phenols was obtained within 8.5 min using only 5 mM p-sulfonic calix[4]arene as additive. Efficiency up to 8.8x105 plates/m was achieved. The influences of running buffer, additive concentration, field strength, temperature, and injection on mobility, elution range, migration of the solutes, resolution, and efficiency were reported, and the interaction of phenols with the calixarene was also discussed.