The first genital tract isolates of Chlamydia A were obtained from parents of babies suffering from TRIC ophthalmia neonatorum, by growth in the yolk sac of fertile eggs (Jones, Collier, and Smith, 1959; Jones, Al-Hussaini, and Dunlop, 1964). It was noted that the fathers of these babies were frequently suffering from non-gonococcal urethritis (NGU) and that the mothers often showed changes in the cervix. The isolation of Chlamydia from two of nine un- selected males suffering from NGU confirmed the association of these organisms with non-specific genital infection (Dunlop, Al-Hussaini, Garland,
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Hilton et al. (1974) studied this question.
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