A pentahexosylceramide isolated from canine kidney and intestine was shown by enzyme hydrolyses, partial acid hydrolysis, permethylation and analysis of the products by gas-liquid chromatography-mass spectrometry, and serological tests to be a Forssman hapten identical in structure with the Forssman glycolipid from horse spleen. The assigned structure was N-acetylgalactosaminyl(α1→3) N-acetylgalactosaminyl(β1→3)galactosyl(α1→4)galactosyl(β1→4)glucosyl-(1→1)ceramide. In the course of this work, mass spectrometry was employed to elucidate the structures of partially methylated alditol acetates derived from hexosamines.
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Sung et al. (1973) studied this question.
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