A new derivative absorption spectrum assay allows for sensitive measurement of amphotericin B in brain tissue, demonstrating its concentration correlates with prolonged incubation in scrapie-infected hamsters.
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Brain amphotericin B levels may guide dosing in prion models; leaves open translation to human therapy.
A simple, sensitive, and reproducible assay for the measurement of the amphotericin B concentration in tissue extracts was developed by using the fourth derivative of the absorption spectrum of amphotericin B between wavelengths of 330 and 430 nm. The amphotericin B concentration in spleen and brain was proportional to the total amount administered. The amphotericin B concentration in the brain was highly correlated with the increase in the mean incubation period of intracerebrally scrapie-infected hamsters.
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Casaccia et al. (1991) studied this question.
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