Structure of bean mitochondrial tRNAPhe and localization of the tRNAPhe gene on the mitochondrial genomes of maize and wheat

Bean mitochondrial tRNAPhe, purified by RPC‐5 chromatography and two‐dimensional gel electrophoresis, has been sequenced using in vitro post‐labeling techniques. It is the first plant mitochondrial tRNA sequenced. It shows 76% homology with bean chloroplast tRNAPhe and has many features characteristic of prokaryotic tRNAsPhe. It was used as a probe to localize the tRNAPhe gene on the mitochondrial genomes of maize and wheat.