THE effect of diluting fowl semen on its fertility has been studied by Bonnier and Trulsson (1939), Gilbreath and Davis (1949), and Weakley and Shaffner (1952); in each case, whatever the diluent, fertility decreased with increasing dilution rate. In some cases, however, this decrease was not apparent until a certain dilution rate was reached. Munro (1938) showed that when a constant number of spermatozoa was inseminated in a varying volume, the effect on fertility of dilution with seminal plasma was very small. When a constant insemination dose was used, so that sperm numbers were proportional to dilution rate, fertility decreased with increased dilution rate. He therefore concluded that fertility was influenced by the number of spermatozoa inseminated, at least when this number was less than 108. Chang (1946), working with the rabbit, showed that when a given number of spermatozoa (30,000–40,000) was inseminated with different volumes of a saline . . .
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Rowell et al. (1957) studied this question.
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