The retention of acids by serum based on the retention coefficient: its use in manometric experiments

THE two-vessel method of Warburg [1924] has the unique advantage for the measurement of tissue metabolism that it permits the simultaneous and con- tinuous reading of respiration and total acid production. Whilst serum may be used as the medium for such measurements [Warburg, 1925], and is a more physiological medium than simple saline solutions, the retention of part of the evolved lactic or carbonic acid caused by its interaction with the buffers other than bicarbonate brings with it the need for special retention corrections.