Why the study?
Uric acid-induced oxidative stress via TLR4-Nox4 signaling contributes to urate nephropathy and renal fibrosis, prompting evaluation of whether Balinese purple sweet potato tuber ethanolic extract could suppress renal Nox4.
Does Balinese purple sweet potato tuber ethanolic extract reduce renal Nox4 levels in mice fed a high-purine diet?
Does Balinese purple sweet potato tuber ethanolic extract reduce renal Nox4 levels in mice fed a high-purine diet?
Balinese purple sweet potato tuber ethanolic extract showed a trend toward decreasing renal Nox4 levels in a high-purine diet mouse model, but the effect was not statistically significant.
PSPEE may attenuate TLR4-Nox4 signaling in hyperuricemic rat kidneys; leaves open any role in human CKD.
Background: TLR4-NADPH Oxidase-4 or TLR4-Nox4 signaling pathway uric acid (UA)-induced oxidative stress is involved in urate nephropathy pathogenesis, which can culminate into renal fibrosis, a common feature of chronic kidney disease (CKD). Based on the hyperuricemic and antioxidant effect of anthocyanin on the kidney, we assumed that Balinese purple sweet potato (Ipomoea batatas L.) tuber in its ethanolic extract form (PSPEE) could suppress the activation of renal TLR4-Nox4 signaling. This study was aimed to investigate the renoprotective effect of PSPEE via renal Nox4 downregulation, using a murine model induced with a high-purine diet.Methods: This study was an experimental laboratory study using a post-test only with a control group design among 38 male Swiss mice. The Male Swiss mice (20-30 g) were randomly allocated into 2 groups, namely, the control group (treated with a high-purine diet for 7 days) and the PSPEE group (treated with a high-purine diet for 7 days and PSPEE for 14 days). The serum uric acid (sUA) level was quantified using the UA phosphatase method. Meanwhile, the renal Nox4 level was quantified using ELISA. Data were analyzed using SPSS version 20 for Windows.Results: Our study found that the renal Nox4 levels were 18.65±1.67 ng/mL and 15.14±1.66 ng/mL for the control and PSPEE groups. Based on the statistical analysis, there was no statistically significant difference between control and PSPEE groups (p>0.05).Conclusion: Balinese PSPEE tends to decrease renal Nox4 level in high-purine diet-treated mice, but fails to show a significant effect.
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Mahendra et al. (2020) studied this question.
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