Why the study?
Does lowering calcium concentration in perfusion media restore beta-adrenergic responsiveness in isolated transgenic mouse hearts?
Does lowering calcium concentration in perfusion media restore beta-adrenergic responsiveness in isolated transgenic mouse hearts?
Lowering extracellular calcium restores beta-adrenergic responsiveness in transgenic mouse models characterized by calcium overload.
Should not inform clinical calcium management; leaves open mechanisms of beta-adrenergic restoration in calcium-overloaded models.
The effects of alterations in calcium in the perfusion media were studied on ss-adrenergic coupling in isolated hearts from 3 different transgenic mice: cardiac-specific overexpressed alpha(1) subunit of L-type calcium channel, overexpressed Galpha(q), and phospholamban knockout. Isolated hearts from all 3 models, when studied at [Ca(2+)] of 2 mmol/L in the perfusate, showed the usual blunted or no response to ss-adrenergic stimulation. Lowering [Ca(2+)] to 0.75 to 1.5 mmol/L unloaded the hearts of calcium and restored to nearly normal the responsiveness to ss-agonist stimulation.
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Serikov et al. (2001) studied this question.
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